
DNA Requirements

Requirements
Oxford Nanopore long-range sequencing has very specific DNA requirements. For submission of DNA to the 1KSA program, the requirements are:
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As much DNA as possible (>1μg of dsDNA as measured by Qubit at a minimum concentration of 100ng/μl)
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Purity ratios as measured by Nanodrop of A260/A280 = 1,8 and A260/A230 = 2 - 2,2
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Average fragment size >10kb as measured by TapeStation, gel or equivalent

Is your research eligible?
Eligibility Criteria
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Your DNA meets our quality standards
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It is preferable if you are affiliated with a South African Institution
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Your organism is indigenous to South Africa
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You have permits and ethics clearance for your specimen
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You agree to comply with the principle of open science and our Terms of Use.
Please review the nominations we've received so far to avoid submitting a duplicate.